Comparison of CyTOF assays across sites: Results of a six-center pilot study.

For more than five years, high-dimensional mass cytometry has been employed to study immunology. However, these studies have typically been performed in one laboratory on one or few instruments. We present the results of a six-center study using healthy control human peripheral blood mononuclear cells (PBMCs) and commercially available reagents to test the intra-site and inter-site variation of mass cytometers and operators. We used prestained controls generated by the primary center as a reference to compare against samples stained at each individual center. Data were analyzed at the primary center, including investigating the effects of two normalization methods. All six sites performed similarly, with CVs for both Frequency of Parent and median signal intensity (MSI) values<30%. Increased background was seen when using the premixed antibody cocktail aliquots at each site, suggesting that cocktails are best made fresh. Both normalization methods tested performed adequately for normalizing MSI values between centers. Clustering algorithms revealed slight differences between the prestained and the sites-stained samples, due mostly to the increased background of a few antibodies. Therefore, we believe that multicenter mass cytometry assays are feasible

Charged particle multiplicity distributions in Z0 hadronic decays

This paper presents an analysis of the multiplicity distributions of charged particles produced in Z0 hadronic decays in the DELPHI detector. It is based on a sample of 25364 events. The average multiplicity is <nch>=20.71±0.04(stat)±0.77(syst) and the dispersion D=6.28±0.03(stat)±0.43(syst). The data are compared with the results at lower energies and with the predictions of phenomenological models. The Lund parton shower model describes the data reasonably well. The multiplicity distributions show approximate KNO-scaling. They also show positive forward-backward correlations that are strongest in the central region of rapidity and for particles of opposite charge. © 1991 Springer-Verlag

Search for scalar quarks in Z0 decays

A search has been made for pairs of scalar quarks (squarks) produced in e+e- annihilations at LEP (√s≃MZ0), and decaying into a standard quark and a neutral, non-interacting, stable, massive particle (the lightest supersymmetric particle, LSP). The search has been conducted for differences in the mass of the squark and LSP of 2 GeV/c2 and above. Up squarks with masses below 42 GeV/c2 and down squarks below 43 GeV/c2 were excluded. Six squark flavours degenerate in mass were excluded below 45 GeV/c2.0SCOPUS: ar.jinfo:eu-repo/semantics/publishe

A Comparison of jet production rates on the Z0 resonance to perturbative QCD

The production rates for 2-, 3-, 4- and 5-jet hadronic final states have been measured with the DELPHI detector at the e+e- storage ring LEP at centre of mass energies around 91.5 GeV. Fully corrected data are compared to O(αs 2) QCD matrix element calculations and the QCD scale parameter ΛMS is determined for different parametrizations of the renormalization scale μ2. Including all uncertainties our result is αs(MZ 2)=0.114±0.003[stat.]±0.004[syst.]±0.012[theor.] .0SCOPUS: ar.jinfo:eu-repo/semantics/publishe

A Search for sleptons and gauginos in Z0 decays

Using a data sample corresponding to 10 000 hadronic Z0 decays, we have searched for the production of sleptons and gauginos in the two-prong decays of Z0. No candidate remains after straightforward selections. For neutralinos, we use selection methods developed in our previous search for neutral Higgs particles. The negative results are translated into improved mass limits and parameter constraints on the minimal supersymmetric extension of the standard model